This data set displays the boundaries of six Brazilian continental biomes: the Amazônia, Cerrado, Caatinga, Mata Atlântica, Pantanal and Pampa. “Biome” is defined as a collection of life (plant and animal) constituted by the grouping of contiguous vegetation types identifiable on a regional scale with similar geoclimatic conditions and shared history, which results in a unique biological diversity. The names used were the most common and popular in general associated with the predominant type of vegetation or relief, as in the case of Pantanal biome, which is the highest provincial flooded surface of the world.